Histo-anatomy and in vitro morphogenesis in Hyssopus officinalis L. (Lamiaceae)

The sequence of histogenesis and organogenesis in the calus of Hyssopus officinalis nodal explants was studied during treatment with growth regulators. This process represents an important indicator of the accommodation capacity of regenerated plantlets to the ex vitro conditions. The cell multiplication rate was stimulated in Murashige and Skoog medium supplemented with indole-3-butyric acid (1 mg L–1). The study was carried out from the start of the culture until the development of shoots. Leaf and stem anatomy was influenced by growth regulators. Combinations between auxine and citokinine have induced callus development at the basis of the regenerated shoots, revealing different degrees of vitrification in stems and leaves.